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Then, 10 L of neutral-red stained zymosan (1 10 8 particles/mL) were added to each well and after 30 min the supernatants were removed and cells fixed with Baker's formol-calcium (4% formaldehyde, 2% sodium chloride, 1% calcium acetate) for 30 min

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The cells were then washed three times with PBS and incubated with Oil Red O working solution (G1260, Solarbio) for 30 min, protected from light