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Quantification of the time course measurements of the fluorescence ratio (F400/488) for HeLa cells with lentivirus mediated LUC and Cr GPDH expression under Dox control transiently expressing iNAP1 ( d ) or inactive variant which does not bind NADPH (iNAPC) ( e ) in the imaging medium (DMEM without pyruvate, fluorescent vitamins and phenol red with 5 mM glucose, 25 mM HEPES, pH 7.4 and 1% dialyzed FBS), or when cells were switched to the basal medium without glucose but with 10 mM pyruvate

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