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With the completion of the reperfusion period, all animals were sacrificed by using a high dose of thiopental sodium (50 mg/kg), as described in previous studies 31,32 , and kidney and lung tissue samples were collected

Mechanistic Research Perspective NNMT enzyme interaction research Cellular metabolism pathway analysis NAD biosynthesis pathway investigation Small-molecule intracellular signaling research Metabolic regulatory pathway studies Primary Research Applications Cellular Metabolism Studies: Researchers use 5-Amino-1MQ to explore metabolic signaling pathways and enzymatic regulation within controlled laboratory environments

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FIGURE 1 Fundoscopy We demonstrated the specific damaging effect of L-NAME application