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Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium (Part No 80026-1)
65°C for 60sec
Removal of small DNA oligonucleotides (e
or for tiling arrays containing overlapping regions of complete chromosomes or genomes
Exonuclease I, 20,000 U 1111 Transformation of Endura Electrocompetent CellsDigest ssDNA, but not dsDNA, in a 3 to 5 direction Specific: Processively digest ssDNA in a 3 to 5 direction without harming dsDNA short 3 overhangs in DNA are not digested Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes Heat Inactivated: Destroy the enzyme activity by incubating at 80C for 15 minutes Exonuclease I digests ssDNA in a 3 to 5 direction,1 3 but does not digest dsDNA. Although
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