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The count data were filtered to remove genes with low expression, transformed using the variance stabilizing transformation from the DESeq2 package (51), and then batch corrected using ComBat (52)

The documented benefits extend beyond theoretical longevity: improved sleep quality and circadian rhythm regulation through pineal gland support, enhanced immune function markers, normalized melatonin production in aging individuals, reduced oxidative stress, improved skin elasticity and appearance, and potential disease prevention through cellular optimization

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2008;13(2):1657 Back M, Yin L, Ingelsson E