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30 l of supernatant was transferred to autosampler vials

Nascimento et al., 2024) mainly by affecting skeletal muscle development ( The RPM supplementation during the last 28 days of gestation promoted an up-regulation in amino acid and glucose transporters in the placentome of cows, mediated by changes in gene transcription and mTOR signaling ( Efforts have been made to evaluate the effect of RPM supplementation during late pregnancy and its long-term effects on offspring performance

The UPLC system was coupled with a high-resolution mass spectrometer using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a HESI electrospray ion source (spray voltage: positive ion mode 4 kV, negative ion mode 3 kV

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