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A standard curve was created using serial dilutions of plasmid DNA containing the target mtDNA sequence, and amplification efficiency was validated with a correlation coefficient above 0.98

Upon activation, STING1 undergoes a conformational change and translocates to the Golgi apparatus, where it activates downstream kinases, including TBK1 (TANK-binding kinase 1) and IKK (IB kinase)

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