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But systemic estrogen levels also matter, and adipose tissue is a significant source of estrogen production through peripheral aromatase conversion

aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF + aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 "10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 "10 M Dihexa (Harding lab) in aCSF, 10 "12 M Dihexa in aCSF or 50 ng/ml HGF + 10 " ' 2 M Dihexa in aCSF for 30 minutes at 37C with gentle rocking

The choice between injections and supplements depends on multiple factors, including the severity of deficiency, underlying health conditions, and individual absorption capabilities

doi: 10.1016/j.jneumeth.2018.03.006