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A simple rotation pattern might alternate between left abdomen, right abdomen, left thigh, and right thigh over a 4-week period

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RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency

Therefore, future research should focus on prospective, multicenter, randomized controlled trials to further verify the efficacy and safety of DPP-4 inhibitors in DCM