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The procedure also delayed Parkinsons by up to several years among those who eventually did develop the disease (Killinger et al., 2018)

Determination lipid peroxidation activity Lipid peroxidation activity was measured in the form of malondialdehyde using thiobarbituric acid based on Stocks and Dormandy method with some modifications [27]

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NAD+/NADH ratio assays, SIRT1 activity assays, and mitochondrial respiration measurements (such as oxygen consumption rate via extracellular flux analysis) are standard methods for studying this combination's proposed effects in cell-based models, typically comparing single-compound and combined-compound treatment conditions against untreated controls