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The protein concentration of metabolite extracts was measured with a bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Waltham, MA, USA) to normalize samples to cell biomass 60

During IS, elevated extracellular glutamate levels (Speer et al., 2013

Raising systemic alkalinity to optimize methylation enzyme activity through alkaline mineral intake (potassium bicarbonate, magnesium, and calcium citrate) and dietary changes

Figure 6 Redox imbalance and oxidative stress Inflammation is bidirectionally linked to redox imbalances (248) as inflammation produces ROS and reactive nitrogen species (RNS), whilst redox imbalances cause cellular damage, evoking an inflammatory response (249, 250)