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( PMC ) Formula by proportion Use these proportions for the finished volume : GHK copper peptide (powder): 0.5% Hyaluronic acid solution: 20% Aloe vera gel: 20% Glycerin: 5% Shea butter extract: 5% Distilled water: 49.5% 50 mL batch example For a full 50 mL batch: GHK copper peptide: 0.25 g Hyaluronic acid solution: 10 mL Aloe vera gel: 10 mL Glycerin: 2.5 mL Shea butter extract: 2.5 mL Distilled water: 24.75 mL Easy scaling formula For any batch size , multiply total volume by these numbers: GHK copper peptide (grams) = total mL 0.005 Hyaluronic acid solution (mL) = total mL 0.20 Aloe vera gel (mL) = total mL 0.20 Glycerin (mL) = total mL 0.05 Shea butter extract (mL) = total mL 0.05 Distilled water (mL) = total mL 0.495 Example for 30 mL : GHK-Cu: 0.15 g HA solution: 6 mL Aloe gel: 6 mL Glycerin: 1.5 mL Shea butter extract: 1.5 mL Distilled water: 14.85 mL Step-by-step instructions 1

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For DDA1 , it was cloned with no-tag or N-terminal GST-TEV tag

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