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This is one of a series of commentaries under the banner 50 years forward, giving personal opinions on future perspectives in diabetes, to celebrate the 50th anniversary of Diabetologia (19652015)

For example, ferroptotic macrophages have the ability to generate HMGB1, a DAMP that triggers the release of pro-inflammatory cytokines, including IL-1 and IL-18, and activates the inflammasome (61)

Diabetic neuropathy: mechanisms, emerging treatments, and subtypes

For the untargeted experiment, proteins were digested with 0.7 g of trypsin (sequencing grade, Promega, Madison, WI) and 0.7 g of Lys-C (New England Biolabs) for 1 h at 37 C