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Description

Membranous glomerulonephritis MGN is one of the most commonly associated GN with the use of cosmetics

M.ZhangW.ZhangC

Proteomics 11, 20192026

Cell pellets (~2030 l of packed cell pellet) were re-suspended in 1 ml buffer containing 20 mM HEPES (pH = 7.5), 10 mM KCl, 250 mM sucrose, 5 mM MgCl 2 , 1 mM EGTA, 1x phosphatase inhibitor cocktail (Sigma, P0044) and 1x protease inhibitor cocktail (Sigma, P8340), and lysed by addition of digitonin (Sigma, D141), 200 g/ml final concentration at 4 C for 10 min