The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature
Influenced by hormones, immunomodulators and metabolic factors, these autoimmune diseases exhibit features of ferroptosis features, including iron metabolism disorder and lipid peroxide accumulation at different stages of development, suggesting that ferroptosis plays an important regulatory role in the onset and progress of autoimmune diseases
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