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Consequently, the GSH deprotonation step was considered non-rate-determining and thermodynamically favoured, leading us to use the GSH-thiolate as the starting point to compute the reaction mechanism for the conversion of flav-3-on-4-ol ( 7 ) into cyanidin ( 8-B4 ) in the wild-type enzyme and the C13S variant (Extended Data Fig

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Funding This work was supported by a National Health and Medical Research Council of Australia Capacity Building Grant and a UNSW Faculty of Medicine Research Grant

This process is similar to how the body handles any dietary protein, converting it into the building blocks it needs for various functions