We employed an adeno-associated virus vector (AAV2/8) to deliver and express either human wild-type hepsin (hHPN WT
Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)
Trad KS, Fox MA, Simoni G, Shughoury AB, Mavrelis PG, Raza M, et al
Fundamentals of Copper Peptide Analysis Understanding Metal Coordination In its most stable form, GHK-Cu forms a square-planar complex in which the copper ion coordinates through nitrogen donors from histidine and the amine groups of glycine and lysine