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Briefly, cells plated at a density of 2 10 5 cells/cm 2 in 12-well tissue culture-treated polystyrene plates, were first grown for 2 weeks in Williams E medium (Invitrogen, Cergy-Pontoise, France) supplemented with 10% (vol/vol) fetal calf serum (FCS) (Cytiva, Velizy-Villacoublay, France), 100 IU/ml penicillin, 100 g/ml streptomycin, 5 g/ml bovine insulin (SigmaAldrich, Saint-Quentin-Fallavier, France), 2 mM glutamine (Invitrogen) and 50 M hydrocortisone hemisuccinate (Upjohn, Paris La Dfense, France)
Syst Rev 10:89
228 Two mechanisms seem to be involved: a) women with anaemia cannot tolerate the same volume of postpartum bleeding as healthy women, and it can be life-threatening in mothers with clinically significant anaemia
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