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Preceding the Von Frey test, mice underwent a 15-minute acclimation on a wire mesh

Within 2-3 days of stopping: Acute sleep-deepening effects fade Sleep quality may feel slightly reduced compared to peak cycle benefits No withdrawal symptoms (DSIP has no documented withdrawal profile) Over 1-2 weeks off: Sleep quality gradually returns toward pre-cycle baseline Circadian rhythm improvements may partially persist the entrained patterns do not disappear overnight Some users report maintained sleep improvement for 7-10 days after stopping This period is useful for assessing the natural sleep baseline Why cycling matters: No tolerance to DSIP has been documented in clinical literature

This framing of intracrinology shows that receptivity testing is not only a calendar problem, but also a local hormone-response and tissue-programming problem, and this is precisely the distinction where multi-omics endotyping may add value

Chem Res Toxicol 31(8):658665