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Introduction Glucosinolates (GSLs) are sulfur-rich secondary metabolites primarily present in Brassicale plants and well-known as important defense compounds that are beneficial to human health ( O -sulfated (Z)- thiohydroximate ester via a sulfur atom and are originally derived from amino acids ( The GSL biosynthetic pathway has been almost completely elucidated in recent decades ( As multifunctional enzymes, glutathione-S-transferases (GSTs) are primarily involved in the conjugation of the tripeptide glutathione (GSH) to the electrophilic center of lipophilic compounds (Supplementary Figure 1) have been identified through multifaceted gene co-expression network analysis ( in silico prediction, and molecular evidence is lacking

19 Cavali M, Ezzedine K, Fontas E, et al

34 , 11511167.e1157 (2022)

Low levels of PAHs and acrylamides the smokeless coffee roasting machines ensure levels are low