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T gel =1.8 (% F127)+1.3 (% F68)+53 An equation is fitted to the data obtained and can be utilized to estimate the Mean Dissolution Time (hr) based on the gelation temperature of F127/F68 mixtures (for 17-25% F127 and 0-10% F68), using results obtained in examples above: MDT= 0.2 ( T gel )+8

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Monitor for symptoms or allergic reactions and discontinue use if any appear

PT-Link (Dako PT100) and EnVisionTM FLEX Target Retrieval Solution Low pH (pH 6.0, K800521-2, Agilent, Santa Clara, CA, USA) were used for slide deparaffinization, rehydration and antigen retrieval