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Abbreviations TP: Tobacco powder TL: Tobacco leaves TSNA: Tobacco-specific nitrosamines B1: Bacillus subtilis B1 C4: Cytobacillus oceanisediminis C4 CMC-Na: Carboxymethylcellulose sodium PDA: Potato dextrose agar PCR: Polymerase chain reactions NCBI: National Center for Biotechnology Information BLAST: Basic local alignment search tool HnB: Heat-not-burn SDE: Simultaneous distillation extraction SEM: Scanning electron microscope XRD: X-ray diffraction FTIR: Fourier transform infrared spectroscopy PCoA: Principle coordination analysis LDA: Linear Discriminate Analysis LEfSe: LDA effect size C4.TP: Cytobacillus oceanisediminis C4.tobacco powder C4.TL: Cytobacillus oceanisediminis C4.tobacco leaves B1.TP: Bacillus subtilis B1.tobacco powder B1.TL: Bacillus subtilis B1.tobacco leaves NMDS: Non-metric multidimensional scaling References Alagi S, Selekcija IS, Pali R, Stojanovi G, Nikoli M (2002) Chemical composition and antimicrobial activity of the essential oil of the oriental tobacco Yaka

Smoking is one of the greatest significant unfavorable factors for precancerous lesions and cancerous diseases of the lips and oral cavity

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