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Neonatal estrogen exposure induces lobe-specific alterations in adult rat prostate androgen receptor expression

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Hair follicle organ culture Briefly, after separation of the hair follicles (HFs) under a binocular dissecting microscope, the proximal two-thirds of anagen HFs located in the subcutaneous fat were isolated using fine forceps (Scitech Korea Inc., Korea), and subsequently collected in a 35-mm culture dish containing complete hair follicle culture medium (Williams E