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Therefore, to determine whether GHK-Cu affects neutrophil infiltration to alleviate ALI, we analyzed neutrophil infiltration into the lung by detecting total MPO, an enzyme released from activated neutrophils, and the neutrophil count in lung BALF

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The animals were placed in an induction chamber with an oxygen-enriched environment, with the initial induction concentration set at 4%5% isoflurane, until deep anesthesia signs such as loss of the righting reflex appeared, after which maintenance was initiated

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