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Following paraffin removal and dehydration, sections were incubated in 3% hydrogen peroxide for 30 min to quench endogenous non-specific peroxidase activity

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The OPTION-DM trial was a multicenter, randomized, crossover study comparing three monotherapy sequences (amitriptyline, duloxetine, pregabalin) against two dual-therapy sequences in patients who had failed one monotherapy [65]