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Disruption of the MTOC results in defects in spindle assembly and subsequent arrest in oocyte development ( 2.4 Oxidative stress leads to dysfunction of the spindle assembly checkpoint The spindle assembly checkpoint (SAC) functions as a pivotal regulatory mechanism within the cell cycle ( 2.5 Strategies to mitigate oxidative stress for the prevention of oocyte deterioration As women age, reproductive senescence is linked to increased oxidative stress within the ovaries