First, the overall gene expression patterns were visualized using principal component analysis (PCA), which revealed evident transcriptomic differences among the PBS, P-Mn@mHSA, and P-Mn/TI@mHSA groups, with no outlier samples detected (Fig
Falkowski M, Maciejczyk M, Koprowicz T, Mikoluc B, Milewska A, Zalewska A, et al
The maximum number of simultaneous overlapping MRM transitions within any given retention window did not exceed 10, which is well within the theoretical and practical limits of the instrument
Flow cytometryintracellular staining Cell samples were washed and stained with Zombie Aqua viability dye (BioLegend, USA), followed by fixation in 2% ROTIHistofix (Carl Roth, Germany) for 15 min