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In brief, brains and spinal cords were rapidly dissected in cold 0.15 M sodium cacodylate pH 7.4 containing 4% paraformaldehyde (PFA) and 0.5% glutaraldehyde

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A binary mobile phase system was used, buffer A: 0.1% (v/v) formic acid (FA) in water and buffer B: 0.1% (v/v) FA in acetonitrile (ACN)