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After 4 hours incubation, the infected cells were probed for mature virus with A12 primary antibody (anti-PV human serum), which binds specifically to mature virus and provirions (Nihal Altan Bonnet, unpublished results), and Alexfluor 488-conjugated secondary antibody (green color)

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However, further studies are required to demonstrate that encapsulation improves the biological efficacy of polyphenols conclusively