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A few things: aggressive agitation (shaking), exposure to the wrong pH, or using a non-sterile diluent that introduces contaminants

Therapeutical interventions that aim to increase GSH concentrations in vivo include N -acetyl cysteine

Supernatants were incubated with CytoTox 96 Reagent for 15 min at room temperature, protected from light, after which stop solution was added

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