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Following three additional TBST rinses (5 min per wash), the cells were incubated with fluorescently labeled secondary antibodies and DAPI for 1 h at room temperature in the dark

RSFCsymptom association analyses To explore the relationship between SCAN-subcortical RSFC and both motor and non-motor symptoms, we performed separate partial least squares (PLS) regression analyses linking RSFC between six subcortical ROIs and the cortical SCAN to each of four clinical measuresMDS-UPDRS-III, MMSE, HAMA and HAMDin the PIPD dataset

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