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The MDA content was calculated as follow: [6.45 (OD 532 OD 600 ) 0.56 OD 450 ] Vt /( Vs m ), where Vt represents the total volume of the extraction solution, Vs the volume of the extraction solution taken during the determination, and m represents the mass of the sample (23)

In our exploratory analyses, we observed a significant condition-by-sex interaction for Glx/tNAA change, with male participants showing more pronounced differences between placebo and naltrexone pretreatment conditions compared to females

We first compared the differences between these two compounds in improving the phenotype of mice with NASH