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Tokushige, K

Intravital fluorescence signals (excitation at 620 nm, emission at 670 nm) were collected at various time points (1, 3, 5, 8, 10, and 24 h) post-injection using an in vivo fluorescence imager (IVIS Lumina III, PerkinElmer, US)

The result is sustained caloric deficit without the metabolic adaptation that makes dietary restriction alone so difficult to maintain long-term

Cell counting After the functional outcome test, the numbers of BrdU+/NeuN+ mature neurons in the Olfactory Bulb (OB) were counted in 1 out of 10 consecutive, adjacent sections from the anterior portion of the OB to the beginning of the accessory OB