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Proper handlinggently rolling, not shaking, the vialis also crucial to preserve the delicate peptide chains

This initial permeability is thought to be due to the sudden hypoxia of different brain cells and early BBB disruption (54)

The NbLumSyt1-HALO/JF549 complex was then centrifuged at 18.000 g for 10 min at RT to remove precipitates, and subsequently diluted (1:200) in High K + buffer, mixed at RT for 5 min in gentle rotation (20 rpm), and used for imaging on the same day

Short-term to mid-term stability was assessed as the percentage of peak area recovery for samples stored in the autosampler at 10 C for 24 h, 3 days, and 6 days, relative to the peak area of the initial measurement