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Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse colon tissue lysates

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A: Ipamorelin binds the GHSR-1a receptor and activates intracellular calcium signaling via the Gq/PLC pathway, leading to growth hormone release

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