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Gliaastrocytes, microglia, and oligodendrocytesare not passive supporters but active regulators of neuronal survival and function

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Saved supernatants and controls were filter-sterilized and analyzed using an Iron Assay Kit (Sigma-Aldrich, MAK025, Ireland Ltd.), Calcium Assay Kit (Cell Biolabs, MET5121, USA), and Magnesium Microplate Assay Kit (Cohesion biosciences, CAK1107), according to the manufacturer's instructions