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Cellular adhesions marked by endogenous paxillin-GFP were labeled with anti-GFP primary antibodies (Life Technologies, Carlsbad, CA, USA, Cat# A-11122, RRID:AB_221569, dilution: 1:500) and Anti-Rabbit Alexa Fluor 488 secondary antibodies (Invitrogen/Fisher Scientific, Goat Polyclonal, Cat# A-11008, RRID:AB_143165, dilution: 1:500) in the m-EGFP-tagged Paxillin (PXN) WTC iPSC line, while the actin cytoskeleton was labeled with Acti-stain 488 (Cytoskeleton, Inc., Denver, CO, USA, Cat# PHDG1-A) in the TagBFP-tagged d-Cas9-KRAB WTC iPSC lines

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10.3390/jcdd8100130 J Cardiovasc Dev Dis

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