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(PubMed) Habeb AM, Flanagan SE, Zulali MA, et al

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The mobile phase A (H 2 O/0.1% formic acid) and mobile phase B (100% acetonitrile/0.1% formic acid) were delivered through a liquid chromatograph nanoElute (Bruker Daltonics GmbH, Bremen, Germany) at a flow rate of 200 nL/min, maintaining the column temperature at 50C