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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten min at 95 C

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Glutathione helps reduce inflammation by neutralizing free radicals unstable molecules that cause oxidative stress and damage cells

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