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Furthermore, when ASS1- deficient mesothelioma cells were treated with ADI-PEG20 and ODC inhibitor DFMO together, synthetic lethality was observed in vitro [149]

The first cDNA was then subjected to PCR amplification to enrich nucleic acids

After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking

Enfoque mecanicista de la investigacin En entornos de laboratorio controlados, el DSIP se estudia habitualmente en relacin con las vas de sealizacin mediadas por pptidos y los sistemas de comunicacin molecular