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The resulting supernatants were diluted in a 10 times volume, and the dilutions were used in the assay

When glutathione neutralizes a free radical, it becomes "spent" or oxidized

Determination of peroxy radical-scavenging of lactic acid bacteria

probiotic treatment significantly suppressed these neuroinflammatory pathways ( Moreover, SCFAs and tryptophan metabolites, as key mediators of bidirectional gut-brain communication, play essential roles in maintaining intestinal barrier integrity, modulating immune function, and transmitting signals via the vagus nerve (Pan et al., 2025)