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Pregnenolone, produced from the CYP11A1 catalyzed side-chain cleavage of cholesterol, is converted to 17-hydroxyprogesterone (17OHP), the universal precursor of cortisol production, by HSD3B2 and CYP17A1 17-hydroxylase activity

At the same time, the remaining part of the liver was soaked in a 10% buffer formalin solution and stored at -20C for later histopathological examination

135 Tirzepatide is developed by Eli Lilly and has proven to provide superior blood sugar control and significant weight loss, making it particularly valuable in the treatment of T2DM