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In vitro transcription Capped mRNA was transcribed in vitro in the presence of the cap analogs m 7 G[5]ppp[5]G or m 2 7,3-O G[5]ppp[5]G (New England Biolabs, Ipswich, MA, USA) using the T7 High Yield RNA Synthesis Kit (New England Biolabs) and DNA templates amplified by PCR from the constructs described above using Phusion Polymerase (New England Biolabs) and the following primers (Additional file 2): 10 and 13 for pIVEX1.3_eYFP-His, pIVEX1.3_His-eYFP, pCITE2a_His-eYFP, pIVEX1.3_FFLuc-His and pIVEX1.3_RRLuc-His

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