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Briefly, LX-2 cells were grown to a confluence of 60-70% on glass coverslips, washed three times with PBS, fixed in 4% formaldehyde for 20 min, permeabilized in 0.5% Triton X-100 for 5 min and blocked with Duolink blocking solution for 60 min at 37 C

Brenzel A, Gressner AM: Characterization of insulin-like growth factor (IGF)-I-receptor binding sites during in vitro transformation of rat hepatic stellate cells to myofibroblasts

Jastreboff AM, Kaplan LM, Frias JP, Wu Q, Du Y, Gurbuz S, et al

Furthermore, miR-155 deficiency led to a reduction in steatosis and fibrosis in MASH despite the presence of significant inflammation