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In particular, we aimed to assess the role of inflammation and oxidative stress with a focus on the nuclear factor-kappa B (NF-B), the Janus kinase/signal transducer and activator of the transcription3 (JAK/STAT3), nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (Nrf2/HO-1), peroxisome proliferator-activated receptor-gamma (PPAR-), and silent information regulator-1 (SIRT1) in pathogenesis of intestinal injury induced by MTX

Mistake 5: Using the wrong syringe or miscalculating doses

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The predominant isoform is the NOX2 in brain endothelial cells, and it has been observed that the Nox2- knockout mice induce less MMP-9 and diminished expression of occludin, a critical protein of the BBB permeability (Liu et al., 2007)