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The AIF pathway, together with increased levels of cleaved caspase 3, is recognized as the main inductor of apoptosis in the damaged brain of neonatal rodents 38 , even if their action becomes less pronounced once the brain matures 39

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An all-in-one Cas9 nuclease reporter vector expressing Cas9 and, including gRNA against GLA and GFP was transfected by electroporation into iPSCs (CMC11), and the cells were then incubated for 710 days