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In organotypic hippocampal slice cultures, Dihexa and Nle-AngIV similarly augmented spinogenesis, with control slices showing 7 spines per 50-m dendrite length, compared to 11 for both Dihexa and Nle-AngIV treated slices [1]

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Since we expect the native-like structures of the fusion proteins to be preserved in the solubilization experiments that we tested, which also bypassed the refolding step (and, saved time), we wanted to ascertain the intactness of the secondary structure

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