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Briefly, isolated crypts were centrifuged for 2 min at 68 g , resuspended in the appropriate volume of cold mouse colonic organoid growth medium (bioGenous, K2204-MC) and embedded in equal volume of Matrigel matrix (ABW, 082703) at 50250 crypts/20-30 L in a 48-well plate

Controlled Substance Hydrocodone bitartrate and acetaminophen tablets contain hydrocodone, a Schedule II controlled substance

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Gustavo Le Paige Archaeological Museum is located in the commune of San Pedro de Atacama, in the II Region of Antofagasta, Chile