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Step 3: Add Water to the Peptide Vial Protocols describe inserting the needle into the GHK-Cu vial at an angle, aiming at the glass wall not directly at the powder and letting the water trickle down the side of the vial gently

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Surrogate variables to correct for batch effects To account for potential batch effects , we performed surrogate variable analysis (SVA) based on the DNA methylation levels at all CpG sites located on chromosome 10 using the R/Bioconductor package sva [31]