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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

Concern and Recommendations: Medically, the major concern right now with BPC 157 is a lack of evidence confirming safety in humans

One hundred units equals one milliliter